جستجوی مقالات مرتبط با کلیدواژه « Syzygium aromaticum » در نشریات گروه « کشاورزی »

The drug-induced liver injury (DILI) has a wide range of clinical presentations, from asymptomatic liver enzyme elevations to cirrhosis. Herbal dietary supplements may be beneficial to reduce the risk of hepatotoxicity. This study aimed to evaluate the effects of different doses of clove extracts on humoral factors in rats with hepatotoxicity induced by thioacetamide.

In this experimental study, rats were divided into nine groups (10 rats per each). The Control group received no treatment. The Sham group was treated with oral administration of distilled water (0.5 ml) for 21 days. The positive control group received thioacetamide (50 mg/kg for three days) intraperitoneally. The clove group was divided into three subgroups and given daily oral administrations of 50, 150, and 300 mg/kg of clove hydroalcoholic extracts (for 21 days). Rats in the experimental group were divided into three subgroups and subjected to 50 mg/kg thioacetamide injection after receiving hydroalcoholic extracts of clove (50, 150, and 300 mg/kg, respectively) for 21 days in the last three days. All rats were sacrificed after 48 hours to measure liver function parameters (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total plasma protein, and albumin).

The rats that received thioacetamide showed liver damage by increased serum liver biomarkers and decreased levels of total plasma protein and albumin compared to the control group. The different doses of clove extract resulted in a significant improvement of liver damage by reduced serum liver enzymes levels and increased total plasma protein and albumin.

Oral administration of the different doses of the clove extract (50, 150, and 300 mg/kg) for 21 consecutive days could significantly improve the changes associated with serum biomarkers of hepatotoxicity.

The importance of using different medicinal plants indicates their health and valuable effects such as antioxidant, antimicrobial, anti-inflammatory effects and etc. In this research, the effect of alcoholic extract and essential oil of Syzygium aromaticum on Escherichia coli was investigated. The effect of the plant on this microorganism as a gram negative was evaluated and important indicator of poisoning and diarrhea at levels of 3, 4, 5, 6, 7, 8 and 9 mg/ml of broth culture media. By dilution method using liquid culture media, minimum growth inhibitory concentration and minimum bactericidal concentration were determined. The results of this study indicated increasing the concentration of extract and essential oil in the studied area increased their antibacterial effect and the most inhibitory effect was on essential oil which means that the minimum inhibitory concentration for ethanolic extract and essential oil was 8 and 6 mg/ml, respectively. Similar results were observed in relation to MBC. Also, by comparing the obtained results, it was deduced that the inhibitory effect of the essential oil used in this study on E. coli was greater than the clove extract and the essential oil of the cloves had a more antibacterial role than extract. Eugenol, eugenol acetate, cavicol and beta-caryophyllene were observed in GC-MS analysis and the difference in the content of bioactive ingredient eugenol further confirmed this antimicrobial effect.