جستجوی مقالات مرتبط با کلیدواژه « Soluble leishmanin » در نشریات گروه « پزشکی »

Delayed-type hypersensitivity reaction measured by skin testing, is an important tool for evaluation of cellular immune response in leishmaniasis and has been used recently as an indication of exposure to infection. Skin testing in leishmania infection needs a standard reagent with high specificity, sen sitivity, and potency. In the present work, a soluble leishmanin, and three whole parasite prepara tions from Leishmania major, i.e. thimerosal, phenol, and autoclaved leishmanins, were prepared un der standard conditions. These antigens were evaluated and compared in different foci of leishmani asis. The sensitivity and potency of reagents were tested in both recovered cases and patients with ac tive ulcer in two areas endemic to urban and rural leishmaniasis. Data obtained showed that, the sensitivity of thimerosal, phenol, and soluble leishmanins are almost equal, but are higher than auto claved one. Moreover, the potency of soluble leishmanin was also proved to be higher than other reagents. The purity of soluble leishmanin is higher than other three particulate preparations, and it lacks the majority of membrane antigens. As a result this reagent can be used as a standard and ideal antigen for skin testing in human leishmaniasis.