epinephelus coioides

Benzo[a]pyrene (BaP) is the most toxic compounds in Petroleum that by contaminating the aquatic ecosystems, weakens the immune system of aquatic animals and ultimately reduces their resistance to opportunistic pathogens. Vibrio alginolyticus is a species of these opportunistic pathogens, Which, in stressful conditions, causes the disease of vibriosis in different organisms. In order to investigate the histopathological changes caused by two stressors including V. alginolyticus and BaP on kidney of Epinephelus coioides, 140 fish were divided randomly in 7 groups for 16 days. The control treatment received no injection and control vehicle received only coconut oil. Also, One group injected with 107 cfu/ml of V. alginolyticus. Groups 4 and 5, respectively received 20 and 200 mg/kg BaP and treatments 6 and 7, respectively injected with (20mg/kg BaP+107 cfu/ml V. alginolyticus) and (200mg/kg BaP+107 cfu/ml V. alginolyticus). All fish were sampled on days 1,4,7 and 14. The tissue sections were prepared, then, were examined under a light microscope. The most histopathological alterations observed in different groups were included formation of melanomacrophage centers, leukocyte infiltration, the separation of the basement membrane from the renal tubular epithelium, destruction of blood cells, glomerular capillary dilation and hemorrhage. The results showed that by increasing the concentration of Benzo[a]pyrene, the amount and severity of lesions increased. The most of the lesions were observed on combined treatment, especially fish treated with 200 mg/kg BaP+107 cfu/ml V. alginolyticus at day 7,so that tissue alterations such as tissue degeneration, necrosis, melanomacrophage centers and hemorrhage were observed in this treatments.

Plasma biochemical parameters are important aspects in the management of endangered species. The values of these parameters can be used for confirming the maturity and for monitoring any changes in the quality of waters and related soils.The aim of this research was to investigate the reference values of biochemical factors in sea bream (Acantopagrus latus) and grouper (Epinephelus coioides). Blood samples (30 grouper and 30 sea bream) were collected from captured fish from coastal waters of Chabahar (Ramin waterfront). Plasma levels of glucose, Blood Urea Nitrogen (BUN), cholesterol (CHO), triglyceride, total protein, albumin, calcium (Ca2), phosphorus (P), sodium (Na), bilirubin and potassium (K) were measured. The plasma levels of different biochemical values in A. latus and E. coioides were Na (328.50± 32.03, 271.90±27.88), K (2.12±0.42, 3.43±0.39), Ca2(19.80±2.97, 16.80±2.34),P (2.35±0.40, 2.40±0.57)(mmol/l)and CHO (257.30±35.51, 137.30±42.11),triglyceride (102.02±11.82, 68.10±15.34), BUN (3.82±0.55,2.66±0.45), bilirubin (0.24±0.04, 0,44±0.13), glucose (44.25±16.18, 43.70±10.11) (mg/dl), protein (4.31±0.32, 3.92±0.36) and albumin (0.15±0.03, 0.36±0.05) (g/dl) respectively. It can be concluded that the values of blood biochemical parameters is may be affected by It can be concluded that the studied parameters may be affected by physiological factors such as the species of fish.

Benzo [a] pyrene is one of the most common and potently carcinogenic polycyclic aromatic hydrocarbons (PAHs). The aim of this study was to investigate the effects of B[a]P on the histological status of the kidney in the Epinephelus coioides. In this study, we investigated the histopathological alterations in the kidney of the E. coioides after exposure to B[a]P. 100 immature protogynous E. Coioides were exposed to different concentrations of BaP (0, 2, 20, 200 mg/kg) for 14 days after one week acclimation period. Six fish were randomly selected from each treatment in 4, 7 and 14 days of experiment. After necropsy, their kidneys were fixed in Bouin‘s solution for 2 weeks. Sections in six-micrometer thickness were obtained and stained with Hematoxylin-Eosin for examination by light microscopy. On the basis of the results, melanomacrophage centers, glomerular capillary dilation, thinking of glomerular basement membrane (GBM) and necrosis were the most common pathological changes in all treatments in all days. The degree of severity and extension of alterations was more in higher doses.