Using Novel Immobilization Engineering Technique to Increase the Efficiency of Antibody Immobilization on the Surface of Nylon and Nitrocellulose Supports, Utilized in Diagnostic Systems

Aim and Immobilization of biomolecules on the surface of different supports, so that it’s physicochemical properties to be maintained to Lead to an increase in process efficiency, is the most essential prerequisite for the design and manufacturing of biological sensors. Antibodies are the most efficient molecules for this purpose. This study was done aiming at increasing the efficiency of immobilization of antibody on nitrocellulose and nylon supports. In this study, different materials and polymer layers such as PEI, MAMEC were used to process (activation and blocking) the surface of nitrocellulose and nylon. Increasing the number of functional groups in nitrocellulose and creating new active groups on the surface of activated nylon, were the most important modifications on mentioned solid supports. After immobilization of antibody on above supports, the process efficiency was assayed and then was compared with control samples. The results showed that the modifications caused increasing of antibody immobilization approximately 2, 3 fold on the nitrocellulose and nylon supports, respectively. this study demonstrated that depending on the kind of solid support, the type of processing may be different; and also it is possible to change the physiochemical features of support like capillarity, flexibility, surface charge, etc which may affect the quality of immobilization negatively.