Effect of H2O2 and Melatonin on In vitro Oocyte Maturation

The aim of this study was to determine the effect of stress oxidative (H2O2) and different concentrations of melatonin on nuclear maturation of immature oocytes in sheep. Ovary collection and oocyte recovery was carried out by standard method. Oocytes culture was in the following conditions: A: TCM199+10% FBS, 5µg/ml FSH, 0.01IU/ml LH, 100 IU/ml penicillin and 100 IU/ml streptomycin, B: A + H2O2 (300 µM) at 38/5 C˚, C: B + 1 µM melatonin and D: B + 10µM melatonin. This study showed that H2O2 significantly (P<0.05) decreases nuclear maturation in compare to control (14.7 vs. 84. 9). 0, 1 and 10 µM melatonin could improve oocytes to reach to metaphase-II stage (respectively 14.7 vs. 43.29, 54.12). But increasing melatonin dose from 1 to 10 µM, did not have any significant effect on oocytes maturation. The results of this study showed that melatonin improves sheep oocytes maturation during oxidative stress.