Localization and Effect of Hyper and Hypo-osmotic Environments on Distribution of Na+, K+- ATPase in Kidney Tubules of Juveniles of (Epinephelus coioides)

Histological study of kidney nephrons of Epinephelus coioides Juveniles and Immunolocalization of Na+, K+- ATPase during osmoregulation and adaptation with hypo- and hyper-osmotic (10 and 60 ppt) conditions. H & E staining and IgGα5 and FITC as primary and secondary antibodies were used for kidney nephrons histological studies and Immunolocalization of Na+, K+- ATPase, respectively. Results of microscopic study indicated that kidney nephrons of juvenile grouper were consisted of Glomerulus, Neck segment, Proximal, Distal and Collecting tubules. Immunolocalization of control group showed that Na+, K+- ATPase was distributed in all part of nephron structure but glomeruli. In hypo- and hyper-osmotic environments similar to control condition the enzyme distributed into the epithelial cells of proximal, distal and convoluted tubules but was not presence in glomeruli. The results of present study indicated that, the structure of kidney nephrons of E. coioides was similarto that of other euryhaline species and presence of Na+, K+- ATPase in basolaterl portion of cell membrane indicated its active role in osmotic and ionic regulation in kidney.