Antibody production against Chlorpyrifos Pesticide (first experience in Iran)

Nowadays use of pesticides are common in agriculture and yearly so many investigations are carried out for determination of pesticidal residue in crops with gas and liquidchromatography. But these methods are expensive and time consuming. Developing of new methods of immunoassays like ELISA are interesting for many researchers. In this research, evaluation of production of antibody against chlropyrifos has been done. Pesticide is broken, then conjucation was made with protein BSA and rabbit got immunized by injection. Then after different intervals the blood was collected and titer was determined with ELISA reader and showed great difference with reference.This method is cheap, easy and more sensitive as compare as chromatography.