Morphological Changes of Small Intestinal in 21 Day-Old Male Broiler Chicks in Response to Addition of β-Mananase and β-Glucanase to Barley- Soybean Basal Diets with Two Metabolizable Energy Levels

This experiment was carried out in order to investigate the effects of addition of β- mananase (M) and β-glucanase (G), alone or in combination (MG), to barley soybean basal diets with two levels of metabolizable energy (ME), Lower (L) or equal (H) of broiler chicks requirement. For this purpose 120 one day-old male broiler chicks were randomly allocated to 8 treatments by 3 replicate (5 birds in each replicate). In day 21, 8-cm segments from their duodenum, Jejunum and ileum were sampled, and then villi length and width and crypts of lieber khon depth of each sample were measured by use of light microscopy and LeicaQwin image analysis software. Data from this experiment were analyzed in form of a 4×2 factorials experiment (4 enzyme combinations and 2 ME levels) in a completely randomized design. Results indicated that ME levels only affected duodenal and jejunal crypts depth (P<0.01). Different enzyme combinations affect duodenal and ileal villi length, duodenal villi width and jejunal crypts depth. HMG and LMG treatments compared with other treatments increased duodenal villi length. Addition of enzymes compared with control groups, increased duodenal villi length in duodenum. Villi width were less affected by treatments, widest villi were for LM (338 μm), HM (368.5μm) and HMG treatments in duodenum and in LMG and HM treatments in ileum. Jujenal villi widths were not affected by treatments. HMG treatment increased crypt depth in duodenum and ileum and adversely decreased it in jujenum.