Evaluation of the exercise effect on morphine-induced toxicity in rat liver and kidney

Increasing evidence on human and animal studies have suggested that exercise has benefits on overall health. It has been shown that exercise stimulated endogenous antioxidant enzymes and reduced oxidative damage markers. Morphine (M) is widely used for relief of severe pain. The mechanism by which M –induced toxicity is due to generation of reactive toxic intermediates. To evaluated the effect of exercise on M produced toxicity in rat liver and kidney Subjects and M was given ip two times per day for 5 consecutive days. 1st day 5mg/kg,2nd day10 mg/kg, 3rd day 20mg/kg, 4th day 40mg/kg and 5th 50mg/kg. Control animals received vehicle only. Treated rats were divided in 4 groups. One group of M and vehicle treated rats were participated in the treadmill exercise sessions (1 h at speed 17 m/min) for 10 consecutive days. and two other group did not participated in the exercise.24 h later, rats were killed with overdose of sodium pentobarbital. Blood was collected for determination of ALT, AST, ALP, BUN and creatinine. Liver and kidney tissues were removed, fixed and processed for light microscopy. 10 animals were used for each group. Biochemical and histopathological observations indicated that M produced injury in the liver, and kidney. Exercise reduced M-induced toxicity (p