Effect Of Aqueous And Hydroalcoholic Extract Of Beberis Vulgaris On Insulin Secretion From Islets Of Langerhans Isolated From Male Mice

considering the use of Beberis vulgaris in traditional medicine as a blood sugar depressant، in this study، the effect of Beberis vulgaris extracts were investigated on the level of insulin secretion from islets isolated of langerhans in male mice. This experimental study was carried out on 90 adult male mice، NMARI strains; weighing 20-25 g. Pancreatic islets from normal mice were isolated by collagenase digestion method. Then the aqueous and hydro-alcoholic extract of Beberis vulgaris at 0. 05، 0. 1، and 1 mg/ml concentrations and glyburide at 1 and 10 μM concentrations were applied on islets isolated in three different concentration of glucose solution (2. 8، 5. 6 and 16. 7 mM). Insulin secretion from hand-picked islets were evaluated in the static incubation system. The level of Insulin secretion was measured by the ELISA insulin kit. Data were analyzed with variance analysis. Insulin secretion was significantly increased at 16. 7 mM glucose concentration in comparison with 2. 8 and 5. 6 mM glucose concentration (p<0. 05). Incubation of pancreatic islets isolated at 2. 8 and 5. 6 mM glucose concentration and low concentrations of extract (0. 05 and 0. 1mg/ml) significantly increased the insulin secretion (p<0. 05). Glyburide at 10 μM concentration was more effective than aqueous and hydro alcoholic extract of Beberis vulgaris at 16. 7 mM glucose. The present study supported the anti-diabetic effect of Beberis vulgaris extracts in vitro with low glucose concentration and it suggests that one of the anti diabetic mechanisms of this plant is via pancreatic islets.