Biological Control of Root Knot Nematode of Tomato Meloidogyne javanica with Trichoderma harzianum BI and Salicylic Acid in Greenhouse and an Investigation of their Effect on Induction of Phenolic Compounds and Total Flavonoids on Tomato

Tomato seedlings were inoculated with T. harzianum BI (106 spores/ml) and salicylic acid (5Mm) other separately or combined together in the greenhouse environment. The effect of salicylic acid was investigated on growth of antagonistic agent in vitro. For an investigation of the effect of salicylic acid and of antagonist on pathogenecity factor, treatment with T. harzianum BI by drenching soil method and salicylic acid by spray method exerted the most effect on decrease of the disease severity in greenhouse, (92% reduction for gall number, 65% reduction for egg mass number and 95% reduction for egg number per egg sac). In the second section of the study the ability of antagonist and salicylic acid on induction of phenolic compounds and on total flavonoids. The level of phenolic compounds and total flavonoids was evaluated in a duration of from 1 to 8 day(s) following inoculation with nematode. The amount of total flavonoids in the integration treatment showed significant difference as compared with control. The amount of total phenol increased from first to forth day after inoculation with nematode, and then it decreased in T. harzianum BI and salicylic acid treatment. The results showed the integration application of chemical inducer, salicylic acid and antagonistic agent, T. harzianum BI to increase the power of biological control in comparison with their separate application.