Induction of Rat Antibody Against Surface Proteins (OmpW) of Vibrio cholerae O1

Cholera is an acute intestinal disease that is caused by some members of Vibrionasea family. Among more than a hundred family members, only Vibrio cholera O-1& O- 139 are pathogens for human and some marine animals. Surface proteins of these bacteria are immunogenic and act as colonization factor. The aim of this study was to evaluate the induction of rat antibody against surface proteins of Vibrio Cholerae O-1 (ompW).

The recombinant antigen was injected to rats according to the standard procedures. After four times of subcutaneous injection, the rat sera were collected and purified using protein G column chromatography. Then the efficiency of antibody response was determinedusing ELISA.

Recombinant protein concentration was measured to be about 0.9 μg/ml. Rat serum response to recombinant protein was positive up to the concentration of 1/25000 in ELISA reaction. Concentrations of purified antibody were estimated about 0.78 μg/ml. ELISA responses for antibody against ompW dealing with bacterial suspension with OD600= 0/5 was assessed positive to 1/25000 while they did not react with other intestinal bacteria.

The recombinant ompW protein was immunogenic and the produced antibody was able to identify whole Vibrio Cholerae O-1 bacteria and did not react with other related bacterial strains.