Investigation of Cell Cycle Gene Expression (Cyclin B1 and CDK-A) Change Induced by Cadmium in Root Tips of Wheat (Triticum aestivum L.) Seedlings

Aim and The toxic effects of cadmium on growth parameters and cell division in Meristem of different plants have been demonstrated. However، the molecular mechanisms of this process are not completely understood until now. The aim of this study was to investigate the effect of cadmium on the expression of CDK-A and cyclin B1 genes in root tip of wheat seedlings for the identification of molecular cadmium limiting mechanism. Treated wheat root tips with 0، 5، 10، 15، 20، 30، 60، 90 and 120 mg/l concentrations of cadmium used for RNA extraction and RT-PCR with primers specific for cyclin B1 and CDK-A genes. Resuls showed that expression of cyclin B1 gene at cadmium concentrations ranging from 5-15mg/l was invariant. But in higher concentrations (more than 15 mg/l) limiting effect of cadmium was quite clear. Also expression of CDK-A gene at 60، 90 and 120 mg/l concentrations of cadmium affected by limiting effect of cadmium. The decrease in root and coleoptiles growth of wheat seedlings treated with different concentration of cadmium depends on the expression of cyclin B1 and CDK-A genes and other unknown factors.