Comparison results of a domestic indirect ELISA for detection of antibodies Toxoplasma Gondeii to the results of a commercial ELISA for detection of antibodies Neospora Caninum

Neospora Caninum is an obligatory intracellular parasite that has similar morphological and biological characteristics to Toxoplasma Gondeii. The researchers have shown similarity between antigens of these parasites. In this study the results of a domestic ELISA for detection of antibodies in cow’s sera to Toxoplasma gondeii was compared to the results of a commercial ELISA for detection of antibodies in same sera to Neospora caninum via calculation of kappa statistic. The agreement between the tests was equivalent to 0. 65 that shows a substantial agreement. Based on these observations، the existence of cross reaction among antibodies against Neospora caninum and Toxoplasma gondeii is possible. So، using the commercial ELISA for detection of antibodies to Neospora caninum should bedone with more precaution and direct diagnosis of the parasite، beside the indirect tests such as ELISA، might overcome the unsatisfactory aspects of ELISA in this study.