Binding ability Of some Iranian Native Lactobacillus spp. to Aflatoxin M1

Aflatoxins are the most important carcinogenic fungal mycotoxins. Aflatoxin M1 transfer to human by consumption of contaminated milk and other dairy products. Nowdays biological methods are concern as a way to eliminate and reduce mycotoxins. One of this methods is by using specific lactic acid bacteria that have an ability of binding to aflatoxins and inhibition of toxin. Methods of research: Ten Lactobacillus spp. isolated from our local yogurt are examed for their ability to bind to aflatoxin M1 in PBS containing 20 ng/ml for 2 h at 37°C by HPLC method. After that, the best strain was selected and for different hours 0, 4 and 24 were examed for its ability to reduce aflatoxin by HPLC. Then heat treatment were done for cells and after 0,4 and 24 h, HPLC tests were done. The binding abilities of Lactobacillus strains ranged from 47.65% to 51.85%.The Y2L5 strain that isolated from Khik yogurt showed the most binding ability (51.26%).Incubation periods had significant effect on the removal of AFM1 by viable cells ranged from 60.76% to 49/9. Reduction of aflatoxin in different periods for heat-killed cells ranged from 51.6% to 57.65%. The results showed that Iranian native Lactobacillus have good potential to reduce aflatoxin. The different incubation periods for removal of aflatoxin have a significant effects for viable bacterial cells but have no significant effects for dead cells