Identification and accurate differentiation of Salmonella enteritidis isolated from Birds breeding and keeping centers of Iran by multiplex PCR

The most common serotypes of Salmonella isolated from infected human subjects are Salmonella enteric serovar Enteritidis. The purpose of this study was to isolate Salmonella enteritidis from feces and carcass of different birds using new multiplex PCR method. Sample studied in this research, were collected from carcasses or feces of 9 days old chickens from North and West aviculturesof Iran and alsoFeces or carcasses of different birds such as golden eagles, swan, geese, Flamengo and etc of several Birds breeding and keeping centersin Tehran. The initial separation of the samples from other bacteria, were performed through microbiological methods For accurate identification of species, the genomic DNA from samples isolated and standard strains of Salmonella by Multiplex-PCR using specific primers were examined. Scrutiny of Colonies using common methods of Microbiology showed that 11.69% of all birds examined, are contaminated with salmonella. After compare and review the results of a Polymerase chain reaction, the genus band was observed in all samples, while only 34.21% of them were Salmonella enteritidis confidently. So the remaining samples are likely to infect other species. Review of Electrophoresis bands of the samples tested, and standard strains with desired size, makes it clear that primers used are proprietary. In addition to reducing time to detect Salmonella enteritidis, also has a high accuracy. Based on these results, M PCR method due to using species– specific primers for Salmonella enteritidis is better, faster and more specific compared with bacteriological methods and differentiation of Salmonella enteritidis and other Salmonella species from other Enterobacteriaceae. Therefore, the use of molecular method M PCR will help to control Salmonella infection and prevent the spread of disease among all the birds