Identification of probiotic properties of native bacillus isolated from poultry farm of Arak

The oral probiotic bacteria with ability to change the intestinal microbial flora play an important role as health supplement in body. After ingestion they are residing in intestinal tract and exhibit beneficial effect. Also probiotic bacteria are suitable alternative for (natural) antibiotics. The purpose of this study was screening of Arak native Bacillus isolated from poultry farms and evaluation of their probiotic properties.Methods of research: In this study 41 samples of fecal material were collected from 8 different poultry farms. After enriching and heat treatment, spore-forming bacteria were screened. The probiotic properties of the isolates (Acid, bile, salt, pepsin, and chicken gizzard extract resistance and antimicrobial compound production) were determined. In this study 140 isolates of Bacillus were screened 14 strains were subjected to more research base on negative haemolysis. Investigation of probiotic properties of isolates showed that most of them could growth in the presence of 10% NaCl, starin 7 was resistance to HCl (up 91.7%) while starins 9 and 10 were completely resistance to bile salts (cholate and deoxycholate sodium). The selected strains were tolerated to chicken gizzard extract and pepsin (up to 80%) and their adhesion ability to intestinal cells in under evaluation. All the strains showed antimicrobial activity against the common poultry pathogens such as Salmonella sp and E.coli. This study showed that the isolates are potential probiotics and after complementary and filed experiment they can be used as poultry probiotics with the ability of inhibition of poultry common disease.