Effect of combination training on nicotinic acetylcholine receptors level in fast- and slow-twitch muscle threads of male Wistar rats

Nicotinic acetylcholine receptors (nAChR) are integral membrane proteins and prototypic members of the ligand-gated ion-channel superfamily، which modify synaptic transmission at the neuromuscular junctions. The Purpose of the present study was to compare the effect of combination training on the content of nAChR in Fast-twitch and slow-twitch muscle fibers of male rats. In this experimental study، a number of 16 male Wistar rats underwent the combination trainings from their 10th week of life. Under the environmental conditions، these animals were randomly divided into two equal groups of control and combination training. Combination training consisted of Strength and Endurance training، which was lasted for 12 weeks simultaneously. Forty-eight hours after the last session of training session، animals were anaesthetized with a mixture of KetamineTM and Xylazine، and their soleus and anterior tibialis were removed. The amount of nicotinic acetylcholine receptors was measured using ELISA kit. In order to compare the effect of training on the given two fibers، independent T test in SPSS 16. 0 statistical software was employed. the difference in the content of nicotinic acetylcholine receptors in the slow-twitch muscle of combination group (3. 49±0. 61pg/mg) was significant compared to control group (1. 87±0. 51pg/mg). In addition، the difference in the amount of Nicotinic acetylcholine receptors in the fast-twitch muscle of combination group (1. 49±0. 42pg/mg) was significant compared to control group (2. 89±0. 51pg/mg; p=0. 001). The content of nAChR is increased by implementation of a combination training protocols in Fast-twitch muscle (tibialis) and slow-twitch (soleus) muscles in the male rat.