Evaluation antimicrobial activity of probiotic bacteria isolated from probiotic products

The most important aspects of probiotic microorganisms are their useful effect on human health. The goal of this paper is to examine the Supernatant antimicrobial ability of separated probiotic bacteria from yogurt and probiotic tablet on the bacterial pathogen agents. In this study, it was isolated probiotic bacteria strains from yoghurt and probiotic tablet, was used and explored with the cooperation af biochimical method. Antimicrobial effects of these bacterial culture supernatants were evaluated on pathogen bacteria strains by using disc diffusion agar and well diffusion agar methods. To reduce the error test were repeated three times and the inhibitory zone diameter was measured, their antimicrobial ability was compared with each other. Six kinds of probiotic bacteria were determined from the sample of yogurt and probiotic tablet. These bacteria had shown a good antimicrobial ability against seven pathogen bacteria. The inhibitoriest effect was shown whit lactobacillus acidophilus of tablets against bacillus cereus according to well diffusion agar with average of inhibitory zone diameter 14mm. Also in the comparison between two method of well diffusion agar and disk diffusion agar, the well diffusion agar was the far more sensitive than disk diffusion agar method. According to this study the produced metabolite from probiotic bacteria can prevent the growth of pathogen bacteria which shows the positive role of this class of bacteia in human health and it would be suggested that is class of bacteria which are abundance in dairies, should be used more.