Study on the polymorphism of insulin-like growth factor 1 gene (IGF1) in West Azerbaijan native chickens

In the present study the polymorphism of promoter region of Insulin-like growth factor 1 gene (IGF1) of west Azerbaijan native chickens was investigated. A number of 100 chickens were randomly selected from west Azebaijan native chickens breeding station and blood samples were collected. DNA was extracted from blood samples in order to amplify a fragment of 813 bp in size from IGF1 gene. The amplified fragments were subjected to restriction digestion with HinfI endonuclease and the resultant digested products were run on 2% agarose gel. The results revealed the existence of two alleles A and C for the examined locus with frequencies of 24.5% and 75.5%, respectively. Three different genotypic variants including AA, AC and CC were identified with genotypic frequencies of 5%, 39% and 56%, respectively. Chi square test based on observed and expected frequencies of different genotypic variants of IGF1 gene showed that deviation from Hardy-Weinberg equilibrium was not significant for West Azerbaijan native chickens. On the basis of obtained results it was revealed that the IGF1 gene had high degree of polymorphism and the relationship between these polymorphisms of IGF1 gene and native chickens economic traits could be assessed in chickens breeding programs