Evidence of Tryptophan at or near Active Site of Glucoamylase I of Arthrobotrys amerospora.
Author(s):
Abstract:
Arthrobotrys amerospora (ATCC 34468) produced glucoamylase in a semi-synthetic medium containing starch as a sole carbon source. Polyacrylamide gel electrophoresis of crude glucoamylase showed three isoenzymes. They were designated as glu I, glu II and glu III according to their electrophoretic mobility. These iso-glucoamylases were purified by column chromatography using DEAE-Sephadex A-50. The major fraction, namely glu I, was subjected to various group specific reagents like NEM, idoacetamide, PALP, DEP, Rose Bengal, NBS and acarbose. N-bromosuccinimide and acarbose totally inhibited glu I. Hg2+ ion did not inhibit glu I activity at 25 m mol concentration. Glu I also showed raw starch activity
Language:
English
Published:
Iranian Biomedical Journal, Volume:3 Issue: 3, Oct - Jul 1999
Page:
103
https://www.magiran.com/p11018