The Molecular Study and Sequence Analysis of Wdhn13 (LEA) Gene in Common, Durum Wheat and Their Ancestral Genome Donor Species

Aim and background. LEA proteins in wheat and cotton were identified and discussed as the first report in late embryonic proteins. Public classification for more LEA genes was inferred from the structure of the protein domain or chemically derived characters. Bioinformatics methods in genome research methods are useful in situations outside the laboratory. Material and Methods. In this study, LEA proteins for Wdhn13 genes in 8 wheat and wild wheat (including Triticum aestivum cv. Sardari, aestivum gonbad, durum Shosh, durum borojerd, urartu, dicocoides, tauschii and speltoides) were used and sequences from Wdhn13 was compared with Single gene sequences of Wdhn13 in NCBI Results. The result of analysis showed that the Wdhn13 gene sequences in Sardari wheat were the most similar to the sequences in NCBI and the Wdhn13 gene sequences in urartu have the lowest similarity to the sequences in NCBI one. This difference is due to amino acid changes in the DNA sequence of samples. BLAST and Phylogenetic tree drawn from DNA sequence based on the UPGMA algorithm also confirmed this result. The Phylogenetic tree drawn with the logical evolution of the gene for the derivation of diploid species, and hexaploid, tetraploid was indicated. The table of sequence also confirmed that the sequence of the BLAST results and Phylogenetic trees are consistent over the time. Conclusion.The main result of this study was that given the amplification of target gene on the genomes of diploid, tetraploid and hexaploid species including genome AA and non-amplification on Species speltoides (BB) and tauschii (DD), it can be concluded that there is a target gene on the AA wheat genome.