A Study on potential microbial removal of diesel oil from contaminated soil in Hamedan city
Message:
Abstract:
Introduction
Environmental contamination with petroleum is an important issue. Diesel oil is a common pollutant. Bioremediation is a new technology to clean up soils. This study was aimed to achieve microorganisms with power of soil cleaning.
Materials And Methods
The bacteria of the contaminated soil with diesel oil were isolated based on morphological and biochemical characteristics. The degradation ability of the isolated bacteria was checked for the consumption of diesel fuel in the minimal salt medium containing different concentrations of diesel fuel (0.1, 0.5, 1 and 3%). Through the checking process, the turbidity due to the bacterial growth with the control tubes was compared.
Results
In the study, 10 different bacterial strains were found. Turbidity measurement results showed that the factors of time, concentration of the pollutant, bacterial strains and interactions between them were significant in all treated samples. In a particular pollutant concentration, as the time passed, the turbidity of the samples was exceeded. The increase rate of turbidity in the concentrations 0.1-1% was more dramatic than the 1-3% concentrations. 3 of the isolated strains in all concentrations caused a higher turbidity than other strains. Discussion and
Conclusion
In the treated samples, the maximum turbidity was caused by the bacterium number 3, which late on, was identified as Pseudomonas aerogenesis. This bacterium was chosen as the best diesel oil degrader of the polluted soils with respect to the climatic conditions of as Hamedan’s.
Language:
Persian
Published:
Biological Journal of Microorganism, Volume:1 Issue: 2, 2013
Page:
77
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