Genetic Diversity within and Between the gazelle populations of Mond in Bushehr and Daime in Ramhormoz using Microsatellite Markers

In this research, four types of fecal have been collected and their DNAs were extracted in order to study the phylogenetic relation and genetic diversity within and among the gazelle populations of mond in Bushehr and Daime in Ramhormoz. Five microsatellite markers including: 33HDZ692, 33HDZ290, 33HDZ974, 33HDZ749 and 33HDZ593 were selected; and Then the PCR reaction, they were amplificated. After amplification, the PCR production were electrophoresed with %8 polyacrylamide gel and after being stained with Silver Nitrate, their genotypes were specified.In this study, all of the five markers were propagated and totally in these two populations, 11 allels were observed for the five markers. In order to study the Hardy– Winberg equilibrium in each markers of each population, the chi– square test was carried out(P< 0.05). The other estimation of population parameters (Na, Ne,Ho,He, I) was done via the POPGENE software version 1.31. The genetic distance between the two populations was calculated through the standard and unbiased Nei genetic distance method(0.36, 0.3080), and the phylogenetic tree was drawn through UPGMA method. Also, the calculation of genetic diversity within and among the population via molecular analysis of variance showed a little genetic diversity among the population (%29).