Determining the genetic polymorphism of Ghashghaei Lak sheep via using microsate llite marker

In this study, complete blood samples of 125 Ghashghaei Lak Sheep of their disperse areas have been prepared.This study has been performed in Kohgiluieh and boirahmad province in 2007. After changing and optimizing salt extraction method, the DNA extraction procedure was accomplished. Then, the quality and quantity of extracted DNA were determined and PCR on DNA samples was fulfilled. In this research, the investigated subjects were tested with two tests of Chi-square and Accurate Testing. In none of tests, the deviation from Hardy-Weinberg balance on the probability of P<0.005 has not observed. In this paper, 7 Alleles were observed for LSCV36 locus as well as 6 Alleles with band range of 130-180 bp for the MCM63 locus; moreover, for MCM63 locus, 6 Alleles with the band range of 94-134bp have been observed of which 4 Alleles were new.Here, the Heterozygosis of LSCV36 locus was observed equal to 0.97; however, the Heterozygosis of MCMA10 locus was estimated about 0.81. The Shanon (H) Information Index of OarVH110 was gained the maximum (2.21) and it was minimum (1.47) for the LSCV36 locus. High average of (H) for all the sites indicated the enormous polymorphism applied to the sites and slight distinction of their own polymorphism which were in balance with the number of Alleles in sites.