Effect of different levels of vitamin C on the Immunological and Hematological parameters in Barbus sharpeyi

In this study the effects of different level of vitamin C in diet on blood factors and stimulation of immune responses of Barbus sharpeyi were investigated. Three hundred seventy five B. sharpeyi (21.6 ±2.26) were divided into five groups in triplicates. Treatments were fed with food supplemented with 0, 200, 400, 800 and 1600 mg.kg-1 vitamin C respectively for 60 days. Blood samples were taken from 5 fish in each aquarium. then hematological (RBC, WBC, Hb, HCV and RBC index) and immunological (Lysozyme activity and serum bactericidal activity) parameters were evaluated in each group. Ten fish in each aquarium were challenged with Aeromonas hydrophila and mortality of each treatment recorded for 14 days. Results showed that no significant differences were seen in PCV and Hb level among the groups (P>0.05).Lysozyme activity significantly increased in groups fed with 400 and 800 mg kg-1 Vitamin C. Serum bactericidal activity didn’t affected with different level of vitamin C supplemented food(P>0.05). After challenge mortality significantly decreased just in fish fed with food supplemented with 800 and 1600 mg kg-1 Vitamin C compare to control. It can be concluded that supplementation of food with 400, 800 and 1600 mg kg-1 Vitamin C can stimulate immune responses of B.sharpeyi.