Isolation and Cloning of Cu/Zn Superoxide Dismutase Genes (AlSOD1 and AlSOD2) From Halophyte Plant Aeluropus lagopoides

The active oxygen species are the most important plant stress agents. Superoxide dismutase is a key enzyme which constitutes the first line of defence against oxygen toxicity. This enzyme is necessary for aerobic life and play an effective role in the process of tolerance to stress and plant longevity. Aeluropus lagopoides is a halophyte plant that tolerate high soil salinity and have high activity of superoxide dismutase enzyme. We used the sequences of SOD genes from another plants in Poaceae family for primer designing and isolated genes from halophyte plant Aeluropus lagopoides. In this investigation two novel SOD genes AlSOD1 and AlSOD2 isolated and cloned in E.coli via pTZ57R/T cloning vector. These two genes encoding 152 amino acids and also have high homology with Cu/Zn SOD Aelurous littoralis, Zea mays, Phyllostachys edulis and Oryza sativa with 92, 89, 88 and 87% respectively.