Effects of plant growth regulators and vitamin combinations on callus induction, somatic embryogenesis and plantlet production of Aloe vera L.

Aloe Vera L. is one of the medicinal plants with different uses in pharmaceutical, cosmetic and food industries. According to increasing demand for the plant species, the most studies of tissue culture have been focused on micropropagation. But somatic embryogenesis as an appropriate way for large scale propagation, production of synthetic seeds and production of secondary metabolites have been ignored. The effect of plant growth regulators including α-Naphtalene acetic acid (NAA) and N6- Benzyladenin (BAP) and vitamin combinations including MS, LS, L2 and modified vitamin were studied, in this work. MS medium with modified vitamin including 0.4 mgl-1 Thiamine HCl plus 0.5 mgl-1 Prydoxine HCl plus 0.5 mgl-1 Nicotinic acid plus100 mgl-1 Myo-Inositol, supplemented with1.0 mgl-1NAA induced 100% callus induction. The most indirect somatic embryogenesis (50%) was induced by applying MS culture medium with modified vitamin supplemented with 0.2 mgl1 NAA and 5.0 mgl1 BAP. Eighty percent of plantlet regenerated from somatic embryos was survived after maturation, plantlet production, and acclimatization.