Comparison of Microwave extraction and hydrodistillation of essential oil from Biebersteinia multifida DC. Conjunction with gas chromatography - mass spectroscopy:Optimization via Box-Behnken

Biebersteinia multifida DC., also known as the “Adamak plant” has been used as a folk medicine for treating muscle disorders and bone damages. The general aim of this project is to extract the aerial parts of the mentioned plant via the microwave method and later comparing this obtained essence with the one produced via water extraction method. Biebersteinia multifida DC essence was gained using two different methods, the microwave and the Clevenger procedure. These samples were afterwards analyzed for their components by using GC device. In microwave method, 15 tests were successfully conducted with Box–Behnken minitab parameters, showing three various levels designed by the software. Later the optimum condition was compared with the Clevenger procedure. The optimum extraction parameters, such as power, Particle source size and the extraction duration were precisely determined via Box–Behnken method to be, 672.7(vat), 0.53and 98.60(minute) respectively. The percentage of the essence exhibited 114.01 % in comparison with the Clevenger method. The R2 parameter belonging to the essential oil percentage was measured to be 96.51, which proves it to be the suitable model. Based on the obtained results it has been confirmed that, although extraction depends on the target compound still the microwave procedure showed to be time-saving with the appropriate extraction yield.