Determination of the best synbiotic between probiotic bacteria Pediococcus acidilactici and prebiotics inulin, oligofructose and xylooligosaccharide

Inability of probiotic bacteria for stable colonization and dominance in intestinal microbiota of aquatic animals، combination of administration with proper prebiotics (Synbiotic) as substrate for increasing growth and stable domination have been suggested. The aim of the present study was determination of the best synbiotic between a probiotic bacteria، P. acidilactici and prebiotics، inulin، oligofructose and xylooligosaccharide. Materials and method s: P. acidilactici was cultured in media containing inulin، oligofructose and xylooligosaccharide as prebiotic. Growth of bacteria and final pH of media were determined in each treatment under anaerobic culture. In addition، short chain fatty acids (Butyric، Propionic and Acetic acid) productions in synbiotic treatments were measured using High Performance Liquid Chromatography (HPLC) system.

Our results revealed that bacteria growth in P. acidilactici with XOS treatments was significantly higher compared to the other synbiotics and to the control groups (P < 0. 05). There were no significant differences between other treatments regarding bacteria growth (P > 0. 05). Moreover، final pH of media in P. acidilactici with XOS group was significantly lower when compared to the other treatments (P < 0. 05). The highest and lowest SCFA produced in all synbiotic treatments were propionic and acetic acid، respectively. While acetic and propionic production showed no significant variation between treatments (P > 0. 05)، the highest production of butyric acid was observed in P. acidilactici with XOS treatment (P < 0. 05). Discussion and

The results of this study confirmed that simultaneous administration of in P. acidilactici and XOS could be a potential optimum synbiotic.