Investigation of the effect of Carbenoxolone in reduction of inflammation severity induced by Salmonella enterica LPS in a mouse model

Heat shock proteins (HSPs) inhibit production of pro- inflammatory cytokines. Carbenoxolone is regarded to induce the production of HSPs. The aim of this study was the evaluation of the effect of Carbenoxolone in reduction of the severity of systemic inflammation induced by LPS in a murine model. %80 lethal dose of LPS was determined in balb/C mice، also appropriate dose of drug for efficient reduction in mortality was determined. LPS+D-GalN with drug were injected to different groups، in different times، after 24 hours the mortality rate was calculated and the severity of necrosis measured in the kidney and livers of the survived mice. After 3 hours serum samples were collected and the concentrations of TNF-α were measured in each group. Administration of Carbenoxolone with LPS reduced the lethality rate from 80 to %20. The severity of necrosis in the liver and kidney of drug administrated groups decreased significantly in comparison with the positive control group. The concentration of TNF-α in mice received LPS increased significantly in comparison with the control group (P=0. 032)، but in drug received groups no significant differences were seen with respect to the control. Discussion and Carbenoxolone significantly reduced the severity and damage due to endotoxic shock.