Determination the effects of natural antioxidants and fat sources on immune system and intestine morphology of heat stressed broilers

This experiment was conducted to evaluate the effects of turmeric powder (TRP), tomato pomace (TP), fat sources (canola oil, soybean oil and tallow) on immune system and jejunum morphometric parameters of heat stressed broiler chickens. Five hundred four one-d-old Ross broiler were randomly distributed to 36 experimental units and 12 dietary treatments (3 replicates with 12 birds in each). The completely randomized design with factorial arrangement 3× 2× 2 (3 oils: canola, soybean, tallow, 2 TRP levels involved 0.4, 0.8% and 2 TP levels 3, 5%) were used. A daily heat stressed schedule (33oC for 5 h) was applied from 29 to 42d. At 28 and 42 d of age, two birds were bled and slaughtered to take jejunum samples. The antibody production against SRBC, IgG and IgM were higher when canola oil, 0.8 % TRP or 5% TP diets fed. The canola oil increased villus height pre heat stressed birds. The villus width was increased when TP diets fed birds(P<0.05). The villus height, crypt depth and villus height: Crypt depth ratios were higher in canola and villus widths were higher in heat stressed broilers fed tallow. The birds fed 0.4% TRP had larger villus width than 0.8% TRP diet. The crypt depth and villus height: Crypt depth ratios were impacted by the interaction between oil, TRP and TP supplementation. Therefore, addition of canola oil, and natural antioxidant could improve the immune system and jejunum morphometric.