Synthesis and antitumor evaluation of spiro quinoxaline-pyrazoline derivative on K562 cell line

Aim and The quinoxaline and pyrazoline derivatives have variety of biological effects، which made them useful pharmaceutical agents، such as cytotoxicity activities، that will be appropriate in chemotherapy of tumors. In this study، 5''-phenyl-2''،4''-dihydrospiro [indeno[1،2-b] quinoxaline-11،3''-pyrazole] compound was synthesized and studied cytotoxic effect on K562 cell line and PBMC by MTT assay. 5''-Phenyl-2''،4''-dihydrospiro [indeno[1،2-b] quinoxaline-11،3''-pyrazole] compound by one pot assay was synthesized and studied anti tumor activity on blood cancer cell line، K562، and healthy human hole blood activated PBMC by MTT assay. In addition، cytotoxic effects of cisplatin on the same cells were studied. This compound showed antitumor activity with IC50 of 0. 92 and 0. 75 µg/ml respectively on K562 cell line and PBMC+PHA. While cisplatin، as a kind of standard drug in chemotherapy، inhibited proliferation of the same cells respectively with IC50، 1. 71 and 7. 8 µg/ml. The obtain results showed compound 4 is cytotoxic more than cisplatin on K562 cell line and activated PBMC by PHA.