Assessing genetic diversity in wild morphotypes of Iranian Triticum boeoticum according to the allelic variation of Glu-1A and Glu-3A gene loci

Genetic diversity in certain morphotypes of wild T. boeoticum collection of National Plant Gene Bank of Iran was investigated according to the allelic variation in Glu-1A and Glu-3A loci using SDS-PAGE electrophoresis of seed storage glutenins. A vast genetic diversity was observed among the studied wheat morphotypes. We scored 8 band patterns consisting of 10 different HMW alleles and 25 band patterns consisting of 13 LMW alleles in Glu-1A and Glu-3A loci, respectively. It was observed that certain alleles have distributed in certain areas and T. boeoticum germplasm collected from East and West Azarbaiejan provinces presented the most number of glutenin alleles. Based on cluster analysis, the studied morphotypes of T. boeoticum populations collected from certain areas of Iran, were divided into three distinct categories including (1) morphotypes collected from West Azarbaiejan, Lorestan, Eilam, Kermanshah and Kordestan provinces, (2) morphotypes collected from East Azarbaiejan and Fars provinces, and (3) morphotypes collected from Zanjan province and those with unknown origin. Plant breeding programs gain of genetic variation in the target germplasm. So, the vast genetic diversity of Glu- 1A and Glu-3A loci in wild morphotypes of T. boeoticum of Iran, introduces the studied germplasm as a valuable source for the breeding of meal quality and other important qualitative and quantitative traits in wheat.