Investigation of oxidative condition after cold shock stress on thermophilic bacterium, Thermus sp. GH5

Thermophils are a group of microorganisms that optimally grow at temperatures above 45 °C. Reduced sulfur and warm condition are essential for their life and have been found in both terrestrial and marine environments which are heated by geothermal activities. Thermophils have special enzymes and metabolic characteristics that are used in biotechnology. Since thermophiles live in stressful environments it will be very useful to study their survival mechanisms. In this study cell extract from bacteria (Thermus sp. GH5، that is isolated from Ardabil (North West of Iran)) in normal condition (growth at 75 °C) and cold shock condition (growth at 45 °C) were compared. Up-regulated antioxidant proteins and the proteins involved in detoxification showed the creation of oxidative condition about 8 hour after cold shock on thermophilic bacterium. It is possible to extract and use of these stable proteins as diagnostic indicators or protein markers in physiological condition or use in the industrial applications.