Dendrosomal curcumin induced apoptosis by suppression of pluripotency genes in 5637 bladder cancer cells

Although the anti-cancer properties of curcumin، the poliphenol extracted from the rhizome of curry، is confirmed by many investigators، however، low level of uptake، tissue distribution and rapid metabolism has limited its application as an anti-cancer drug. This study is aimed at increasing curcumin water solubility due to biodegradable، neutral and non-toxic micellar nano-carrier called dendrosome as well as evaluating the role of dendrosomal-curcumin (DNC) on bladder cancer cells growth using MTT assay، Flowcytometry، AnnexinV-FLUOS (apoptosis detection kit). Genetic mechanism of DNC-induced apoptosis was accomplished through study of relative expression of OCT4A، OCT4B1، SOX-2 and Nanog using Real-time PCR. The Results indicated DNC-induced cells death complies with a time and dose dependent paradigm in 5637 cell line. Cell cycle analysis revealed that the number of cells increased in pre-G1 and gradually decreased in G1 and S phases. This is indicative of inhibitory property of dendrosomal-curcumin on DNA synthesis. Data from Real-time PCR showed the expression of OCT4A، OCT4B1، SOX-2 and Nanog can be related to 5637 cancer cells growth. Dendrosomal-curcumin significantly suppress the mRNA expression of above mentioned genes (pvalue<0. 01). All together، these data showed DNC induced apoptosis by suppression of pluripotency genes in 5637 bladder cancer cells and confirm the useful character of nano-drug in bladder cancer therapy.