Study of allergenicity of petal and stamen in middle-aged ontogenical stage of Cytisus scoparius L. in guinea pig

Cytisus scoparius is an ornamental and highly aromatic plant. The petals and stamens in aromatic plants are important allergenic agents. The aim of this study is examination study of allergenicity of petal and stamen in middle-aged ontogenical stage of Cytisus scoparius L. in guinea pig. Methology: In this experimental study، 9 guinea pigs from hartly stirps were randomly selected and divided into three equal groups. Plant extracts were prepared with 16% concentration and injected intraperitoneally to guinea pigs. The first group was considered as negative control (injected with phosphate buffered saline). The second and the third groups received petal extract and petal + stamen extract respectively. In addition intraperitoneal injections within 4 weeks، once per week، subcutaneous injection was performed in the fifth week. Finally، a week after the last injection was taken blood sampling directly from the heart of animals، and the number of eosinophils، immunoglobulin E and blood sugar levels were measured in experimental groups and analyzed data. Skin test (Created wheal diameter) and serological tests (levels of immunoglobulin E، eosinophils، and blood glucose) in both groups treated with Cytisus scoparius L. significantly increased compared to controls. Electrophoretic profiles was observed about 4 protein bands (2 light protein bands and 2 pale protein bands) in the range of 27 to 85 kD in the middle-aged petal and about 6 more specific protein bands in the range of 10 to 75 kD in middle-aged petal whit stamen proteins. The results showed that petal of ontogenical stage of middle-aged in Cytisus scoparius have allergenicity property and and this effect is exacerbated when they are together with stamens.