BACTERICIDAL EFFECTS OF EUCALYPTUS GLOBULUS EXTRACTS ON INTRAMACROPHAGE BRUCELLA ABORTUS S99

Brucellosis is a worldwide zoonotic disease that caused by Brucella spp. They are facultative intracellular pathogens which replicate inside macrophages and in non professional phagocytes cells. The experiences show that medicinal plants can be used successfully to treat bacterial diseases. The aim of this study is evaluation of bactericidal effects of Eucalyptus globulus extracts on intramacrophage Brucella abortus S99. In this study aquatic، ethanolic and acetonic extracts of Eucalyptus globulus leaves prepared. Then effect of extracts on intramacrophge surviving of B. abortus S99 studied on cell culture of Balb/c mice peritoneal macrophages. Therefore، after lysis of macrophages، preparation of serial dilution and culture on Muller-Hinton Agar media، number of grown CFU counted. Results indicated that Eucalyptus globulus extracts have antimicrobial activity on intramacrophage B. abortus S99 after 24h. Overall cell culture results indicated that Eucalyptus globulus extracts have bactericidal activity on intramacrophage B. abortus S99 and ethanolic extract has the most effective antimicrobial activity on intramacrophage brucellae.