The qualitative and quantitative analyses of the effective components of two medicinal plants (Echinacea purpurea and Ononis spinosa) using high performance liquid (HPLC) and thin layer chromatography (TLC) techniques

The purpose of this research is the analysis and identification of the effective components in two kinds of medicinal plants, i.e. Echinacea purpurea and Ononis spinosa. The aerial parts of this plant possess branch-shaped trichoms, which have adhesive secretions containing flavonoid combinations. The subspecies of this plant, called Ononis Spinosa Subspleiosperma, grows in many regions of Iran such as Isfahan, Kohkeloyeh, and Markazi. The plants analyzed in this study were gathered in June from Dasht region in Markazi. After extracting the essence and conducting the separation fractions by the chromatography column based on TLC and Hplc; the rutin, hyperosid, and chlorogenic acid compounds (forms of flavonoid compounds) were identified in this plant which is considered an indigenous species of Iran. The quantity of the identified materials was significant in comparison to Echinacea Purpurea.