Determination of Antimicrobial Resistance Pattern and Production of Extended-Spectrum Β-Lactamases amongst Esche-richia coli and Klebsiella pneumoniae from Clinical Isolates
Message:
Abstract:
Background
The prevalence of antibiotic resistance among extended-spectrum β-lactamase (ESBL) producing Escherichia coli and Kleb­siella pneumoniae has been increased markedly in recent years. The present study was done to know the prevalence of ESBL production among isolates of E. coli and K. pneumoniae and to study the susceptibility pattern of isolates against different antibiotics.
Methods
Extended-spectrum β-lactamase producing E. coli and K. pneumoniae were isolated from various samples ob­tained from outdoor and indoor patients of the Prathima Institute of medical sciences, Andhra Pradesh, India. They were tested for ESBL production by double disc synergy test and resistance to various anti­biotics like fluoroquinolones, cephalosporins, aminoglycosides and ß-lactamase inhibitor combinations and susceptibility to carbapenems were determined by Kirby-Bauer disc diffusion method.
Results
A total of 94 ESBL producing isolates were obtained. Of them 60 were E. coli and 34 K. pneumoniae. They were obtained from urine, sputum, pus, wound swabs blood & tracheal aspirates. Urine (38.29%) was the main source of ESBL-producing isolates from all patients, fol­lowed by sputum (34.04%). About 37.23% of these isolates were col­lected from medical wards and 27.65% were collected from outdoor. All isolates were susceptible to imipenem. The resistance to cephalos­porins (1-4 generations) was almost 100%. Resistance to Aztreonam, Ampicillin and Co-amoxyclav was also 100%. A high degree of resis­tance was observed to other antibiotics.
Conclusion
The highest prevalence of resistance to ESBL in E. coli and K. pneumonia is associated with a multitude of infections in hospita­lized patients with a significant longer duration of hospital stay, in­creased morbidity and greater hospital charges. Advanced drug resis­tance surveillance and molecular characteristics of ESBL isolates is ne­cessary to guide the proper and judicious antibiotic use.
Language:
English
Published:
Journal of Medical Bacteriology, Volume:1 Issue: 3, 2012
Page:
17
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