Effect of aquatic extract of Achillea millefolium on sperm and in vitro fertilization in adult rats treated with Cyclosporine A

In this study we aimed to evaluate the protective effect of aqueous extract of Achillea millefolium on sperm parameters in vitro fertilization against cyclosporin A –induced embryo toxicity. Cyclosporin A is an immunosuppressant drug widely used in organ transplantation to prevent rejection. cyclosporin A reduces the activity of the immune system by interfering with the activity and growth of T cells. Achillea millefolium, a widely distributed medicinal plant, is highly regarded for its medicinal activities, including antioxidant and anti-inflammatory properties. In this study, 44 adult Wistar rats were divided into 4 groups as control, Cyclosporine (30 mg/kg), Achillea millefolium (150 mg/kg) and Cyclosporine – Achillea millefolium group (30mg - 150 mg/kg). At 21 and 45 days Sperm characteristics and fertility Evaluated. The Cyclosporine treated group showed significant (P<0.05) decrease in the sperm count, sperm viability and in vitro fertilization. Achillea millefolium co-administration caused an increase in above-mentioned parameters. These findings manifested that Achillea millefolium aqueous extract may be partially protective against Cyclosporine -induced embryo toxicity