Immunohematological reactions to stressful situations have been interested of many investigators. The aim of this study was to investigate the effect of an intense anaerobic workout session on some parameters of athletes. In this analytic descreptive cross-sectional study، 20 professional athletes in volleyball field with mean age 5 ± 25 years were invited purposefully to study. In this study، 30 second Wingate test was used to assess anaerobic power. Samples of peripheral blood before، immediately after and two hours after exercise were collected and levels immunohematological indices were determined. The results of this study showed that immunohematological cells such as leukocytes، lymphocytes، neutrophils، erythrocytes and platelets counts before، after and two hours after practice، have changed significantly (P <0. 01). Results showed that one session of intense anaerobic exercise has significant effect on immunohematological indices of athletes.