Effect of explants types and levels of plant growth substances on callogenesis in Levisticum officinale Koch. on a modified MS medium

Over time, once again there has been a significant growth in growing and producing medicinal plant species. The biotechnological developments have far-reaching implications in conservation and propagation of endangered species, as well in the genetic improvement of medicinal plant. The genetic variation occurred in calli holds tremendous potential for increasing production efficiency of valuable secondary metabolites. Levisticum officinale Koch. is considered as one of the oldest medicinal plants and spices from Apiaceae, whose root has a diuretic agent for the treatment of kidney stones and kidney and urinary tract infections, reported in many credible pharmacopoeias. In present investigation, callus cultures of lovage explants (hypocotyl, root, leaf, petiole, crown node) were initiated in modified MS medium, called AM1, supplemented with different phytohormonal combinations. After one month, the percentage and rate of callogenesis were analyzed as a completely randomized factorial design. The highest callus growth and best appearance were obtained by hypocotyl and root explants in the AM1 medium supplemented with 2,4-D (1.5 mgl-1) + Kin (0.5 mgl-1) + NAA (0.025 mgl-1).