Investigating the Synergic Effects of Ethanolic Extract of Allium sativum L and Electromagnetic Field with Low Frequency on Angiogenesis in Chick Chorioallantoic Membrane (In Vivo)

A number of researches show anti-proliferative effects of EMF on animal cells. In addition to antioxidant properties of garlic،it contains compounds that can inhibit cell proliferation. This study wasconducted to investigate the effects of garlic extract and EMF with low frequency on angiogenesis. Eighty fertilized eggs were divided into the control group، sham-exposed 1 (treated by normal saline)، sham-exposed 2 (in vicinity of switched off EMF)، experimental group 1 (treated by concentration 100 μg/ml of garlic extract)، experimental group 2 (treated by concentration 200 μg/ml of garlic extract)، experimental group 3 (in vicinity of EMF with intensity of 50 gauss)، experimental group 4 (treated by concentration 100 μg/ml of garlic extract and EMF with intensity of 50 gauss)، experimental group 5 (treated by concentration 200 μg/ml of garlic extract and EMF with intensity of 50 gauss). On the 8th day of incubation، gelatin sponge was placed on the chorioallantoic membrane and experimental groups 1، 2، 4، and 5 were treated with 10 microliters of garlic extract. On the 10th day of incubation، experimental groups 3، 4 and 5 were exposed to EMF for 4 hours. All samples were photographed on the 12th day of incubation and the length and numbers of vessels were analyzed by ANOVA and Tukey statistical tests. The mean number and length of blood vessels in all experimental groups were significantly decreased compared to the control group (p<0. 05). Garlic has inhibitory effects on angiogenesis. Moreover، these effects wereamplified by EMF with intensity of 50 gauss.