Agrobacterium rhizogense - mediated transformation of Atropa belladonna

Atropa belladonna is one of the most important medicinal plants in Solanaceae family. It contains tropani alkaloids that often are synthesised in root. Present study was conducted in order to induction of hairy root in A.belladonna using gene transfer via Agrobacterium rhizogenes method. The stem explants with Agrobacterium suspensions harbouring rolB gene were inoculated. Then, in order to direct regeneration, five compounds (MS, MS + 3 mg/l Kin, B5, MS+ 12 mg/l Kin, B5+ 5 mg/l Kin)) in a completely randomized design with three replications was used. The statistical analysis indicated that among treated hormone, 12 mg /l KIN level in MS medium showed the most shoots direct regeneration. Two methods for the analysis of transgenic plants, phenotypic (observed in hair roots of transgenic seedlings), gene amplification of the rolB (using PCR) was used.