PCR detection of Listeria monocytogenes in bovine endometritis from Shahrekord, Iran

Endometritis as a risk factor for cystic ovarian disease, anoestrus and other reproductive disorder, is characterized by impact of dairy production performance and causes serious economic losses. Listeria monocytogenes can cause endometritis. Considering the importance of what mentioned present study was conducted to detection of L. monocytogenes in bovine endometritis from Shahrekord, Iran. Altogether 180 vaginal discharge samples of bovine endometritis were collected with sterile cotton swabs from Shahrekord, Iran. The swabs were placed directly into Listeria enrichment broth. Secondly enrichments were streaked on Palcam agar. Suspected colonies were evaluated for detecting L. monocytogenes by polymerase chain reaction (PCR) method. L. monocytogenes was not found in any samples. Present study suggests absence of L. monocytogenes in bovine endometritis in the region.