Enhancement of the resistance of mesenchymal stem cells against killing conditions by the oxidative preconditioning

About 70% of MSCs die in the early stages of transplantation into the infarcted myocardium. Several solutions have been made to address this problem. In the last decade، preconditioning of MSCs with oxidative stresses has gained a lot of attention. In this study، we have investigated the effects of preconditioning with hydrogen peroxide (H2O2) on the survival of MSCs and their resistance against oxidative stresses. Mesenchymal stem cells from bone marrow have been cultured. Cells from the passage four were treated with 5، 10، 15، 20، 30، 40، 50، 60، 80، and 100µm concentrations of H2O2، and were then recovered with the fresh medium. Finally، the treated cells were exposed to 500 µM H2O2 as the killing condition. The percentage of survived cells was analyzed by the MTT assay kit. Preconditioning with 5 and 10 µ M H2O2 significantly increased the resistance of MSCs against the apoptosis induced by 500 µ M H2O2. Preconditioning of MSCs with oxidative stresses enhances their survival; therefore، it can increase the efficacy of transplantation.