The effect of interference of Nicotine and immobility stress on performance pituitary- adrenal axis in mature male rats

Nicotine is the alkaloid used by millions of people around the world through smoking and at the same time these people also exposed to some kind stressors that effect on body including endocrine system. The aim of this study was to investigate the interference of nicotine and immobility stress on plasma levels of hormones of ACTH and corticosterone in mature male rats. In this empirical research study, we used 70 mature male Wistar rats were enrolled as the control, sham and experiment groups randomly. 3 experimental groups of different types received doses of 0.3, 0.5 and 1 mg/kg BW nicotine, and 2 experimental group, one received for 2 hours immobility stress and other for 2 hours immobility stress and other plus nicotine with doses of 0.3 mg/kg BW and the sham group received 1cc of saline intraperitoneally for 5 days. 24 hours after the last injection, mice were bled from the heart and were measured hormones ACTH and corticosterone levels. The data were evaluated using ANOVA and Tuky test. The results showed that the nicotine at different doses and immobility stress and immobility stress with nicotine increases plasma levels of ACTH and corticosterone hormones. Discussion and According to the result of this study it can be concluded that the effect of immobilization stress and nicotine enhances the stimulatory effects of nicotine and stress on corticosterone and ACTH