Effects of hydatid cyst antigen on Hella cells in vitro

The prevalence of hydatid cyst in patients with cancer is lower than those of normal population. In this study the effect of different extractions of hydatid cyst antigens on growth of Hella cancer cells has been investigated in vitro.

In this experimental study، crude protoscolices antigen، protoscolices ES antigen and hydatid fluid antigen were prepared and fractions of these antigens were using salting out method. These fractions were added to Hella cell line and incubated for 48h. Then the number of alive and dead cells were counted in comparison to appropriate control. Analysis of data was carried out using SPSS. ver. 16 software and Jonckheere–Terpstra Test. P<0. 05 was considered as significant..

In cell cultures treated with mentioned fractions،all 3 fractions significantly decrease the growth of Hella cancer in comparison to control cell culture. Also fractions of crude protoscolices antigen significantly increase the growth of Hella cancer cells.

Different extracts of hydatid cyst antigens inhibitory affects on Hela cell’s growth in culture medium. Further work is recommended an anti-cancer properties of these antigens.