Evaluation of serum ionized calcium of Makouie breed of sheep in the west Azerbaijan of Iran

To obtain the normal amount of calcium in blood plasma of makouie breed of sheeand evaluates the resistance of this breed considerable decline of this mineral incomparison to other breeds. Makouie sheep can be introduced as a virgin and resistantalpine sheep for breeding in same areas by similar situations. Animals-331 healthy adultmakouioe sheep. 147 heads was male and 157 samples were female. Procedure-After acomplete physical examination of sheep and ascertaining of health, they sampled. For thissampling vacuum tubes or syringe 20 cc and needle no.18 were used to obtain the bloodfrom superior vena cava. No anti clotting agent was used during sampling. All of sampleswere numbered and details (age,sex, date of experiment) were recorded. Consequently,samples were transferred to laboratory to centrifuge and detach the cellular section fromthe serum of blood. In this study quick manual micro calorimetric method wasperformed. Results-Regarding to six degree of freedom, it was no significant relationbetween calcium amounts and age (P>0.05). Calcium amount was 9.42(mg/dl). Thisamount was 8.18(mg/dl) in spring and 7.85(mg/dl) in winter and finally it falls to thelowest point in summer, means: 7.62(mg/dl), (P≤0.05). Additionally, serum ionizedcalcium amount had no relevance to gender but this amount was more in female in allseasons. Regarding to histogram chart, dispersion of data was normal and average ofcalcium purity in this breed sheep was:8.29+0.1668(mg/dl). Donkan test, chi-Squre andMSTATC software was used in this investigation.